Our ultrasensitive immunosensors are based on principles of two fully developed technologies, enzyme-linked immunosorbent assay (ELISA) and self-assembly monolayer (SAM). Despite the high accuracy of ELISA, it is a complex procedure and usually takes hours to complete. In order to simplify the procedure and shorten the turnaround time from hours to 15 minutes, we have developed antibody modified self-linkable nanozyme that functions as detected antibodies, secondary antibodies and enzyme of oxidation to H₂O₂ and TMB for color development. Also, for the purpose of commercialization, our nanozyme was designed to be more stable than HRP used in ELISA. It can be stored at room temperature for at least 10 months and is not affected by pH value changes in various sample types. Meanwhile, to increase the sensitivity and eliminate background signals when compared to ELISA, we chose glass vials to be our containers and fashioned the unique glass fabrication on the surface of the glass by adapting SAM technology. Ultimately the SeraParma™ testing platform combines the high accuracy of an ELISA and the convenience of a rapid test.